Author: Robert Sheaff, PhD, and Ian Mitchell
Abstract
This study investigated whether quantum field exposure generated by Quantum
Upgrade based on Leela Quantum technology, influences adenosine triphosphate
(ATP) production in human cell lines. This double-blind experiment was performed
using A549 human lung carcinoma cells and immortalized human diploid fibroblasts
(HDFs). ATP was quantified using the CellTiter-Glo assay following remote quantum
field treatment applied from over 500 miles away. Treated cells exhibited transient but
statistically significant elevations in ATP production, ranging from 20 to 29 percent. The
findings suggest that non-local quantum field exposure may modulate cellular
bioenergetics. Additional replication and mechanistic work are required to confirm these
observations and elucidate underlying pathways.
Introduction
Adenosine triphosphate (ATP) serves as the universal energy currency of the cell,
driving nearly all anabolic reactions, signal transduction pathways, and mechanical work
within living systems. Because ATP is continually utilized and replenished, its steady-
state levels provide a sensitive indicator of the cell’s bioenergetic balance.
Leela Quantum Tech has developed a proprietary technology designed to project
quantum energy fields capable of interacting with biological systems. Quantum Upgrade
functions through subtle informational fields rather than electromagnetic or mechanical
signals. These fields carry highly coherent energetic patterns that interact with the
body’s intrinsic quantum communication systems. This interaction appears to help
restore biological coherence and optimize cellular and systemic regulation. The system
works by providing a stable coherence field that the body can resonate with to support
balance and overall well-being.
Materials and Methods
Two human cell lines were used: A549 lung carcinoma cells and human diploid
fibroblasts (HDFs). Cells were cultured in DMEM with standard supplements at 37 °C in
a humidified incubator with 5% CO₂.
An independent, double-blind experiment was conducted. Two groups of
cells—randomly labeled “A” and “B”—were prepared identically; one was exposed to
the quantum field remotely (>500 miles away), while the other served as the control.
Neither the experimenter nor staff knew which group was treated until analysis
completion.
Measurements were conducted in quadruplicate, and statistical analysis was performed
using ANOVA with p < 0.05 considered significant.
Results
A549 and HDF cells were distributed into two separate 96-well plates specifically
designed to allow cell attachment. The use of attachment plates ensured proper
adherence of both cell types prior to analysis. To prevent fluctuations in pH that could
occur with repeated removal of plates from a CO₂ incubator, the experiment was
performed at 37 °C in the absence of CO₂. For these conditions, L15 medium with or
without glucose was used, as it is formulated for culture under non-CO₂ conditions.
Following plating, cells were incubated for seven hours at 37 °C to allow attachment,
confirmed by microscopy, but not long enough for cell replication. After incubation, the
plates were randomly labeled “A” and “B” and transferred to a colleague who performed
the quantum exposure procedure in collaboration with Quantum Upgrade based on
Leela Quantum Tech personnel located more than 500 miles away. One plate was
exposed to the quantum field (“treated”), and the other served as the control
(“untreated”). This stage of the experiment was performed entirely in the absence of the
principal investigator to preserve a double-blind design. Within five minutes, both plates
were returned to the incubator, and at predefined time points, ATP levels were analyzed
using the CellTiter-Glo® (CTG) luminescent assay applied directly to each well.
Measurements were performed in quadruplicate at each time point to improve data
quality and statistical reliability.
A transient but statistically significant increase in ATP levels was observed in the
treated cells compared with the untreated controls. This increase occurred in both the
presence and absence of glucose, indicating that the observed effect was not restricted
to a specific metabolic pathway. In A549 cells, ATP levels increased by approximately
20 – 29 %, while HDF cells also showed a transient elevation in ATP concentration,
particularly in the glucose-containing condition.
Although the signal in HDFs was less pronounced than in A549 cells, the trend toward
higher ATP production in treated samples was clearly evident. The removal of the “spill-
over” effect—previously identified as possible cross-influence between charged and
uncharged plates—likely contributed to the stronger response observed in this
optimized configuration, where untreated cells remained entirely unexposed to the field.
Discussion
The results of this experiment indicate that exposure of human cells to a remotely
applied quantum field generated by Quantum Upgrade based on Leela Quantum Tech
technology was associated with a measurable, transient increase in ATP production.
Both A549 and HDF cell lines demonstrated this response under carefully controlled,
double-blind conditions, suggesting that the phenomenon is reproducible across
metabolically distinct cell types.
The increase in ATP levels observed in treated cells, ranging from approximately 20 to
29 percent, reflects a biologically meaningful modulation of cellular energy metabolism.
The fact that similar changes were detected under both glucose-containing and
glucose-free conditions suggests that the effect is not dependent on a specific metabolic
pathway. Instead, it may represent a generalized enhancement of cellular energy
production or efficiency, potentially involving mitochondrial or enzymatic regulation.
The experiment was designed to minimize confounding factors that could influence ATP
measurements. Conducting the study in a non-CO₂ incubator prevented fluctuations in
pH caused by repeated removal of plates, which are known to affect cellular metabolic
activity. The use of attachment-compatible plates also ensured consistent cell
adherence, a critical factor for fibroblast metabolism and reproducible ATP
quantification.
The findings suggest that remote quantum field exposure can transiently modulate
cellular bioenergetics. Possible explanations could involve modulation of mitochondrial
function, transient shifts in membrane potential, or resonance effects influencing
enzymatic activity within the oxidative phosphorylation system. These possibilities
remain speculative and require targeted investigation using direct mitochondrial and
molecular assays.
The double-blind structure of the study, together with consistent replication across two
human cell lines, supports the reliability of the observed response. Future work should
address these gaps by including larger sample sizes, detailed statistical validation, and
independent replication in additional laboratories.
Conclusion
Under rigorously controlled, double-blind laboratory conditions, exposure of human
A549 and HDF cells to a remotely generated quantum field produced by Quantum
Upgrade based on Leela Quantum Tech technology was associated with a transient
increase in ATP levels. The response was observed in both glucose-containing and
glucose-free environments, indicating that the effect was not limited to a specific
metabolic pathway. The magnitude of change—approximately 20–29 percent above
control levels—suggests a measurable modulation of cellular bioenergetics.
While the exact mechanism remains undetermined, the findings provide preliminary
evidence that quantum field exposure may transiently influence energy production in
living cells. These results warrant further investigation using expanded sample sizes,
detailed statistical evaluation, and independent replication to confirm reproducibility and
explore underlying biophysical pathways.
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